Characterization, prevalance and antimicrobial susceptibility pattern of bacterial uropathogens isolated from pregnant women at Lahore general hospital, Lahore, Pakistan
Rabia Habib, Muhammad Danish Mehmood, Sana Noreen, Huma Anwar, Mehreen Gul, Nazia Ayub and Almas Raza
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Urinary tract infection (UTI) is common in ladies living in developing countries which may progress to complications such as pyelonephritis and preterm delivery during pregnancy. The present study provides an insight for causative agent of UTI, their prevalence in pregnant ladies and its association with age, metabolic disorder and gestational period. Total of 375 midstream samples were collected from pregnant women, pure culture were segregated on selective media and identified through analytical profile index (API) to evaluate prevalence of uropathogens in UTI and ASB patients. Isolated uropathogenic E. coli were further characterized by polymerase chain reaction (PCR) using specific primers for genotype cjrA, cjrB, and cjrC. Among 375 midstream urine samples of pregnant women, 160 cases of UTI and ASB (≥105 CFU) were recorded. API analysis of such samples showed 65(40.6%), 55(34.35%) and 40(25%) of E. coli, Enterococci and Staphylococci respectively. Furthermore, PCR based characterization of E. coli revealed 42(64.61%) prevalence of both cjrA and cjrB genotype in asymptomatic and urinary tract infected patients. Prevalence of uropathogen in UTI suspected patients was found significantly higher in 20-40 age group 77(74.75%), diabetic patients 90(87.37%) and women reported in first trimester of gestation period 52(50.48%). Antibiotic susceptibility test results revealed that PCR confirmed uropathogenic E. coli was highly sensitive to Ceftriaxone, Amikacin, Nitofurantoin, Gentamicin and Ciprofloxacin with percentage of 79.2%, 76.4%, 75%, 61% and 52% respectively. Whereas, these isolates were resistant to Ampicillin 89%, Cefuroxime 70%, Amoxicillin 65%, Tobramycin 43% and Ceftazidime 22%.