Carcinoma cervix is the most common cancer among women in developing countries and most common cause of female mortality. Agiogenesis plays an essential role in tumor growth and invasion. CAM assay has been widely explored to study tumour angiogenesis and effect of anti-angiogenic drug. Itraconazole, an anti-fungal drug has high anti-angiogenic activity.
Aim of the study: To study of effect of angiogenesis on cervical intra epithelial lesion and carcinoma using chick chorioallantoic membrane (CAM) assay and the effect of itraconazole on angiogenesis.
Materials and Methods: This is a prospective cross sectional study conducted at Department of Obstetrics and Gynaecology, at D. Y. Patil Hospital and Research centre, Kolhapur over a period of 2 years. 100 patients between ages of 21-65 years with abnormal cervix on visual inspection were included for study.
Results: High grades of angiogenesis were found in this order: Carcinoma, CIN III, CIN II, and normal tissue. After giving Itraconazole drug average angiogenic score was reduced in CIN and Carcinoma.
angiogenesis, carcinoma cervix, chorioallantoic membrane, itraconazole From the study, this was concluded that although the result proves itraconazole to be a prominent anti-angiogenic and anti-cancer drug, there are limitations in the study due to less number of samples. Further in-depth research is more randomised samples are needed to validate these findings.