Background: Urge incontinence is considered when a patient suddenly have an urge to void urine, in this situation there is an unnecessary contraction of the urinary bladder that leads to leakage of urine despite the closed bladder by holding sphincter muscles.
Objective: This study aimed to assess the association between urinary and vaginal microbiomes using 16S rRNA gene sequencing in those with urge urinary incontinence.
Patients and methods: A case-control study in which one hundred women who attended outpatient clinic of Baghdad Teaching Hospital during period from January 2021 to December 2021. In this study vaginal swabs and catheterized urine samples were collected from fifty women who proved to have urge urinary incontinence and another fifty samples were taken from controls. Cusco speculum high vaginal swab taken from all wall of vagina and after that under aseptic technique, folly’s catheter inserted and midstream urine collected. The collected specimens transport to laboratory for genetic study, where these specimens, examined by two genomic DNA isolation and real Time PCR for qualitative detection of urinary and vaginal bacteria.
Results: There was no significant association regarding age and body mass index in both groups (p=0.883 for age and p= 0.9 for body mass index). Enterococci contributed 49% of an individual’s urinary and 45% of their vaginal samples with moderate positive correlation (r=0.564) and significant association (p=0.005). Gardnerella contributed 31% of an individual’s urinary and 24% of their vaginal samples with moderate positive correlation (r=0.463) and significant association (p=0.001). E coli were 14% of an individual’s urinary and 12% of their vaginal samples with strong positive correlation (r=0.822) and significant association (p=0.001). Other bacteria that had weak correlation and non-significant association were Urealyticum 26% urinary and 15% vaginal, (r=0.184) (p=0.214). S aureus 3% urinary and 0% vaginal, (r=0.172) (p=0.246). Finally, M hominis was 10% urinary and 4% vaginal, (r=0.241) (p=0.101). Conclusion: Positive associations between vaginal and urinary genera in urge urinary incontinence. E coli had high correlation between urine and vagina while other microbiomes as Gardnerella and Enterococci have moderate correlation and other microbiomes (Urealyticum, S aureus and M hominis) had weak correlation between urine and vagina in urge urinary incontinence.